PREPARATIVE HPLC SEPARATION OF SUGARS EXTRACTED FROM NON- EDIBLE PORTION OF RIDGE GOURD (LUFFA ACUTANGULA) AND THEIR IDENTIFICATION BY MASS AND TLC ANALYSIS
Keywords:Sugar extraction, ridge gourd, UV inactive, HPLC, ELSD, Separation, LC/MS, TLC.
A rapid, sensitive extraction method was developed using the mixture Methanol-Dichloromethane-Water (MDW) (0.3:4:1v/v/v) and MeOH-H2O phase was assayed for sugar analysis. Photodiode-array detection (DAD) has been used to prove the extracted compound is UV inactive, Highperformance liquid chromatography (HPLC) with Evaporative Light Scattering Detector (ELSD) coupled to electrospray ionization mass spectrometric (ESI-MS) detection in the positive ion mode gave MS and MSn fragmentation data which were employed for their structural characterization. The various standard sugars were spotted using the solvent system n-butanol - acetone - diethylamine - water (10:10:2:6, v/v/v/v) in the cellulose layer for TLC analysis which indicated the presence of lactose, galactose, glucose and xylose. This is the first assay of the sugar profile of the ridge gourd, which can be further developed for characterization and evaluation of their quality with regards to their sugar composition.