SPECTROSCOPIC AND STATISTICAL ANALYSIS ON CANCEROUS HUMAN BREAST TISSUES
Keywords:
FTIR spectroscopy, breast tissues, ductal carcinoma, amide I and amide III bands, Trend analysisAbstract
The sensitivity of FTIR spectroscopy for biomolecular changes is used to classify benign and malignant breast tissues. The analysis is carried out for
a collection of 43 samples which were histopathologically identified as normal, hyperplasia, fibro adenoma, ductal carcinoma and invasive ductal
carcinoma tissues. In the present chapter the infrared spectra of human breast tissues were analyzed in the frequency range between 1100 cm‐1 and
2100 cm‐1.The selected areas indicate the amide I and amide III bands corresponding to vibrational modes of the peptide bonds of the secondary
structure of proteins. Some remarkable spectral differences were observed among normal, hyperplasia, fibro adenoma, ductal carcinoma and
invasive ductal carcinoma tissues. The significant absorption bands which differentiate the different cancer grades were found to be at 1260 cm‐1,
1304 cm‐1,1446 cm‐1 ,1657 cm‐1, 1746 cm‐1 2028 cm‐1 ,2062 cm‐1 ,2084 cm‐1. The difference in the absorbance values or the various cancer grades is
compared using the Trend analysis. Thus it implies that FTIR spectroscopy is useful for the diagnosis of cancerous breast tissues.
