SPECTROPHOTOMETRIC DETERMINATION OF ARSENIC IN BIOLOGICAL SAMPLES USING 2 ACETYL5CHLORO THIOPHENE 5AMINO1,3,4THIADIAZOLE2THIOL
Keywords:Spectrophotometry, Arsenic, 2‐Acetyl‐5‐chlorothiophene5‐amino1, 3, 4‐thiadizole‐2‐thiol, Biological samples.
Analytical application of 2‐acetyl‐5‐chlorothiophene5‐amino1,3,4‐thiadizole‐2‐thiol (ATT) is described for the direct non‐extractive
spectrophotometric determination of Arsenic. The reagents react with arsenic, in acidic medium (pH 6.0., sodium acetate‐acetic acid buffer) to form
light yellow colored1:2 (M: L) complexes. The colour reactions are instantaneous and absorbance values remain constant for over 24 h. The molar
absorptivity and Sandell’s sensitivity of ATT methods are found to be 1.45 X 10 4 L mol‐1 cm‐1 and 0.0040 µg cm‐2 of AsIII respectively. The systems
obey Beer’s law in the range of 0.123‐2.537 µg/ml of AsIII. Since ATT method is more sensitive it was applied for the determination of Arsenic in