DEVELOPMENT AND VALIDATION OF RAMIPRIL ESTIMATION FROM CAPSULES USING VISIBLE SPECTROPHOTOMETRIC METHOD
Keywords:ACE inhibitor, Beer’s Law, Estimation, Folin reagent, visible Spectrophotometry.
A simple, rapid, sensitive, extraction free and cost effective visible spectrophotometric method has been developed for the determination of
Ramipril in bulk and capsule formulations. The proposed method is based on the formation of yellowish brown coloured species by the drug with
Folin reagent and exhibits גּ max at 456.5 nm. The calibration graph is linear over the concentration range of 16‐48µg/ml with Molar absorptivity of
4.98783X103 l/mol/cm and Sandell’s sensitivity of 0.08351 µg/cm2 0.001 abs. unit. The proposed method is applied to commercial available
capsules and the results are statistically compared with those obtained by the UV reference method and validated by recovery studies. The results
are found satisfactory and reproducible. The method is applied successfully for the estimation of the Ramipril in capsule formulations without the
interference of excipients.