RPHPLC METHOD FOR THE ESTIMATION OF ESCITALOPRAM IN BULK AND IN DOSAGE FORMS
Keywords:Escitalopram, RP‐ HPLC, Phosphate buffer, Acetonitrile and Methanol.
The present study indicates a simple, accurate and precise RP‐HPLC method for the estimation of Escitalopram in bulk and in pharmaceutical
formulations. The mobile phase used was phosphate buffer with pH 7.0 and an organic mixture solvent (acetonitrile and methanol in the ratio of 1:1
v/v). Then the mobile phase was prepared by mixing buffer solution and mixture of organic solvents in the ratio of (55: 45 v/v) respectively. The
specification of the chromatographic system 150 mm ×4.6 mm Xterra RP 18, 5 μm, flow rate 1.2ml/min, detection 238nm, injection volume 10μl
and run time 10 min. Only very few HPLC procedures have been reported in the literature for the determination of Escitalopram in pharmaceutical
formulations and biological fluids. There are no reports for the determination of Escitalopram by HPLC in pure form. Hence I have made an attempt
to develop a HPLC method for the determination of Escitalopram in bulk and in pharmaceutical formulations.