DNA INTERACTION STUDIES, ANTIMICROBIAL AND ANTHELMINTIC ACTIVITY OF SULFONAMIDES OF 1, 3DIOXOLANE AND 3,4DIHYDROQUINOLIN2(1H)ONE ANALOGUES: SYNTHESIS AND CHARACTERIZATION
Keywords:3,4‐dihydroquinolin‐2(1H)‐one, antibacterial activity, anthelmintic activity and DNA interaction.
A series of sulfonamide group containing compounds were synthesized by the reaction of 2‐dimethylaminomethyl‐1,3‐dioxolane, I and 7‐hydroxy‐
3,4‐dihydroquinolin‐2(1H)‐one, II with appropriate substituted sulfonyl chlorides, IIIae. The structural elucidation of these compounds is based on
IR, 1H‐NMR, mass spectral data and elemental analysis. The synthesized compounds were tested for antimicrobial activity by disc diffusion method.
Anthelmintic activity of the compounds has been tested on earthworms and few of the compounds show moderate activity. The DNA binding of the
compounds IVa and Va with CT‐DNA has been performed with absorption spectroscopy, which reveals that both the compounds show less binding
propensity towards CT‐DNA. Also the nuclease activity of complexes IVa and Va with plasmid DNA (PUC 19) was studied using agarose gel
electrophoresis. The compound Va can act as effective DNA cleaving agent when compared to compound IVa resulting in the nicked form of DNA
under physiological conditions. The gel was run both in the absence and presence of the oxidizing agent