DEVELOPMENT OF SIMULTANEOUS SPECTROPHOTOMETRIC METHOD OF AMOXICILLIN TRIHYDRATE AND RANITIDINE BISMUTH CITRATE IN SAME DOSAGES FORM

Abstract

Amoxicillin trihydrate (AMOX) and Ranitidine bismuth citrate (RBC) are widely used for the treatment of H.Pylori. infection. These drugs are aimed
for bacterial cure and are recommended for patients with relapsing or complicated duodenal and gastric ulcer. Among the numerous estimation
methods for simultaneous estimation viz, derivative spectrophotometery, area calculation, the simultaneous equation method is very simple and
does not require complicated mathematical calculations. Being rapid and simple UV spectrophotometery is one of the most extensively used
analytical technique for estimation of both the drugs. The absorptivity was found approximately same for all the concentrations and hence both the
drugs obeyed Beer’s law in 2‐20 μg/ ml concentration range. The high values of correlation coefficients (r2) indicated good linearity of calibration
curve for both the drugs. Sandell’s sensitivity μg/ cm2/0.001/abs unit of RBC and AMOX was found to be sufficient and these shows that very less
amount of both drugs can be effectively detected by this method. The recoveries of RBC and AMOX from the standard mixture solution were found
to be 100.44% and 99.83% respectively. The recovery results indicated that RBC and AMOX could be quantified by this procedure simultaneously.