• RAJESH K. PATEL Department of Chemistry, SGS Govt. PG College, Sidhi, MP, India
  • P. K. SINGH Department of Chemistry, SGS Govt. PG College, Sidhi, MP, India
  • RAJESH PANDEY Department of Biochemistry, APS University, Rewa, MP, India
  • BABBULAL PATEL Department of Chemistry, SGS Govt. PG College, Sidhi, MP, India
  • SANDEEP MISHRA Department of Chemistry, SGS Govt. PG College, Sidhi, MP, India



Amino acid, High-performance liquid chromatography, Bovine milk, Singrauli


Objective: The present work deals with the concerned study to determine the influence of variable season on amino acid evaluation from 90 bovine milk collected (March 2018 to February 2019) samples belongs to four tehsils of Singrauli district.

Methods: An aliquot of hydrolysate of collected bovine milk samples were injected into the column (Shim-pack ISC-07/S1504 Na) of the higher performance liquid chromatography-based amino acid analyzer.

Results: Most abundant amino acids were glutamic acid followed by proline and leucine. Average amino acid content comparison, results, in the rainy season, maximum amino acid content was in Deosar and minimum in Singrauli, In winter amino acid is higher in Waidhan and minimum in Deosar and maximum amino acid content was recorded intended for Waidhan and minimum for Deosar in summer. Concentration of amino acid in cow milk was found to be in order of glycine<histidine<alanine<methionine<isoleucine<phenylalanine<threonine<tyrosine<serine<lysine<valine and aspartic acid<arginine<leucine<proline<glutamic acid. Amino acid status in buffalo milk sample was found in order Glycine<histidine<methionine<alanine<phenylalanine<isoleucine<threonine<arginine<tyrosine<serine and lysine<aspartic acid<valine<leucine<proline<glutamic acid. All the amino acids are evaluated as per the mean (n = 6) mean±SD.

Conclusion: It is concluded that cow and buffalo milk samples contained all the amino acids but differed in the contents of certain amino acids. Such variation is purely biological and genetic and is intended for the nourishment of the young. The current investigation would be valuable for the dairy processing industries to formulate nutritionally improved milk-based functional products for a vulnerable segment of the population.


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How to Cite

PATEL, R. K., P. K. SINGH, R. PANDEY, B. PATEL, and S. MISHRA. “AMINO ACID STATUS OF BOVINE MILK UNDER SEASONAL VARIATION AROUND SINGRAULI DISTRICT”. International Journal of Chemistry Research, vol. 5, no. 3, July 2021, pp. 1-4, doi:10.22159/ijcr.2021v5i3.170.



Research Article