• EDEWOR THERESA IBIBIA Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria
  • AGBOOLA PETER OBALOLUWA Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria
  • AMUDA MUTIU OLASUNKANMI Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria
  • MMUO AGATHA IJEOMA Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria
  • OWA STEPHEN OLUGBEMIGA Department of Biological Sciences, Landmark University, Omu aran, Kwara State, Nigeria



Parinari curatellifolia, Phytochemicals, Phenolic, Flavonoid, Antioxidant, Antibacterial, Elements, GC-MS


Objective: Parinari curatellifolia is a medicinal plant that possesses multifunctional properties. The aim of this research was to evaluate the mineral, total phenolic and flavonoids contents and some biological parameters of the methanolic leaf extract.

Methods: The leaves were extracted with methanol to obtain the crude extract. Phytochemical screening was carried out using the method described by Harborne, 1999. The extract was subjected to GC-MS analysis, Aluminum colorimetric assay was used to determine the total flavonoid content while Folin-Ciocalteau reagent was used to evaluate the total phenolic content. The antioxidant activity was determined using the DPPH radical scavenging assay. The mineral content was determined via atomic absorption spectrophotometer. Disc diffusion method was used for the antibacterial assay.

Results: The phytochemical screening revealed the presence of tannins, saponins, flavonoids, terpenoids, alkaloids and cardiac glycosides, while steroids, anthraquinones and cyano glycosides were absent. The GC-MS profile showed catechol as the most abundant phytochemical and the identified phytocomponents were mainly polyphenolics, carbonyls, fatty acids and fatty acid esters. The extract possesses total flavonoid and phenolic contents of 13.46±0.02 mg/g quercetin equivalent and 58.43±0.15 mg/g Gallic acid equivalent, respectively. The extract exhibited a significant antioxidant of 84%. Concentration of the macro and micro elements were quite high in some cases. Na had the highest concentration of 42522.70±2146.50 µg/g, while Cu has the lowest value of 4.18±0.03µg/g. The antibacterial activity of the extract was comparable to that of the standard antibiotic. Bacillus subtilis exhibited the highest zone of inhibition of 29±0.10 mm while Salmonella typii and Kliebsiella pneumoniae gave the lowest value of 14±0.12 mm.

Conclusion: Parinari curatellifolia leaves possess tannins, saponins, flavonoids, terpenoids, alkaloids and cardiac glycosides. It has high antioxidant and antibacterial capabilities.


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Huttner A, Harbarth S, Carlet J, Cosgrove S, Goossens H, Holmes A. Antimicrobial resistance: A global view from the 2013 world healthcare-associated infections forum. Antimicrob Resist Infect Control. 2013;2:31. doi: 10.1186/2047-2994-2-31, PMID 24237856.

Roca I, Akova M, Baquero F, Carlet J, Cavaleri M, Coenen S. The global threat of antimicrobial resistance: science for intervention. New Microbes New Infect. 2015;6:22-9. doi: 10.1016/j.nmni.2015.02.007, PMID 26029375.

Yao X, Zhang T, Tang H, Wang H, Chen W. Free radical-scavenging, nitrite-scavenging, and N-nitrosoamine formation inhibitory activities of extracts from Kunlun composite tea (Coreopsis tinctoria Nutt.). J Food Nutr Res. 2015;3(9):587-92.

Ogbonnia SO, Nkemehule FE, Anyika EN. Evaluation of acute subchronic toxicity of starchytarpheta angustifolia (Mill.) extract in animals. J Biotechnol. 2009;8(9):1793-9.

Tochukwu E. Anti-bacterial effect of different fractionations of acetic acid leaf extract of parinari curatellifolia. Br J Pharm Res. 2013;3(2):195-201. doi: 10.9734/BJPR/2013/1772.

More G, Tshikalange TE, Lall N, Botha F, Meyer JJ. Antimicrobial activity of Medicinal Plants against oral microorganisms. J Ethnopharmacol. 2008;119(3):473-7. doi: 10.1016/j.jep.2008.07.001, PMID 18672045.

Maroyi A. An ethnobotanical survey of medicinal plants used by the people in Nhema communal area, zimbabwe. J Ethnopharmacol. 2011;136(2):347-54. doi: 10.1016/j.jep.2011.05.003, PMID 21575701.

Olaleye MT, Amobonye AE, Komolafe K, Akinmoladun AC. Protective effects of Parinari curatellifolia flavonoids against acetaminophen-induced hepatic necrosis in rats. Saudi J Biol Sci. 2014;21(5):486-92. doi: 10.1016/j.sjbs.2014.06.005. PMID 25313285.

Gbile ZO, Soladoye MO. Vernacular names of Nigerian plants (Yoruba). 2nd ed. Ibadan: Forestry Research Institute of Nigeria; 2002.

Harborne JB. Phytochemical methods. A guide to modern technique in plant analysis. London: Chapman & Hall; 1999. p. 60-6.

Halilu ME, Yebpella GG, Hassan LG, Achor M. Preliminary screening, antibacterial and elemental analysis of the leaves and root bark of Parinari curatellifolia planch. ex benth. (Chrysobalanaceae). Continential J Pharm Sci. 2010;4:1-5.

Peni J, Elinge CM, Yusuf H, Itodo AU, Agaie BM, Mbongo AN. Phytochemical screening and antibacterial activity of Parinari curatellifolia stem extract. J Med Plants Res. 2010;4(20):2099-102.

Doughari JH. Antimicrobial activity of tamarindus indica linn. Trop J Pharm Res. 2006;5(2):597-600. doi: 10.4314/tjpr.v5i2.14637.

Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem. 2001;49(11):5165-70. doi: 10.1021/jf010697n, PMID 11714298.

Kelledar SG, More HN. Estimation of tannins in different parts of Memecyclon umbellantum burm. J Pharm Res. 2010;3(3):554-6.

Anandhi D, Srinivasan PT, Kumar GP, Jagatheesh S. Influence of flavonoids and glycosides from Caesalpinia coriariae (Jacq.) wild as antibacterial compound. Int J Curr Microbiol Appl Sci. 2014;3(4):1043-51.

Setzer WN, Setzer MC. Plant-derived triterpenoids as potential antineoplastic agents. Mini Rev Med Chem. 2003;3(6):540-56. doi: 10.2174/1389557033487854, PMID 12871157.

Heisler I, Sutherland M, Bachran C, Hebestreit P, Schnitger A, Melzig MF. Combined application of saponin and chimeric toxins drastically enhances the targeted cytotoxicity on tumor cells. J Control Release. 2005;106(1-2):123-37. doi: 10.1016/j.jconrel.2005.04.006, PMID 15935506.

WHO. Trace elements in human nutrition and health. Technical report series. Geneva; 1996. p. 119-205.

Robertson DG. Metabonomics in toxicology: a review. Toxicol Sci. 2005;85(2):809-22. doi: 10.1093/toxsci/kfi102, PMID 15689416.

Fernie AR, Trethewey RN, Krotzky AJ, Willmitzer L. Metabolite profiling: from diagnostics to systems biology. Nat Rev Mol Cell Biol. 2004;5(9):763-9. doi: 10.1038/nrm1451, PMID 15340383.

Kell DB, Brown M, Davey HM, Dunn WB, Spasic I, Oliver SG. Metabolic footprinting and systems biology: the medium is the message. Nat Rev Microbiol. 2005;3(7):557-65. doi: 10.1038/nrmicro1177, PMID 15953932.



How to Cite

IBIBIA, E. T., A. PETER OBALOLUWA, A. M. OLASUNKANMI, M. A. IJEOMA, and O. S. OLUGBEMIGA. “TOTAL PHENOLIC, FLAVONOID AND MINERAL CONTENTS OF THE METHANOLIC LEAF EXTRACT OF PARINARI CURATELLIFOLIA PLANCH. EX BENTH”. International Journal of Chemistry Research, vol. 7, no. 2, Apr. 2023, pp. 13-18, doi:10.22159/ijcr.2023v7i2.218.



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