GREEN SYNTHESIS AND CHARACTERIZATION OF ISOLATED FLAVONOID MEDIATED COPPER NANOPARTICLES BY USING THESPESIA POPULNEA LEAF EXTRACT AND ITS EVALUATION OF ANTI-OXIDANT AND ANTI-CANCER ACTIVITY

MARIMUTHU GOKUL; UMARANI G.; AYYAMPERUMAL ESAKKI

doi:10.22159/ijcr.2022v6i1.197

Authors

MARIMUTHU GOKUL Madurai Medical College, Madurai, Tamilnadu, India 625020 https://orcid.org/0000-0003-3935-7755
UMARANI G. Madurai Medical College, Madurai, Tamilnadu, India 625020
AYYAMPERUMAL ESAKKI Madras Medical College, Chennai, Tamilnadu, India 600003

DOI:

https://doi.org/10.22159/ijcr.2022v6i1.197

Keywords:

Antioxidant(s), Apoptosis, Cancer, Nanoparticle(s), Particle size, Spectroscopy, X-ray powder diffraction (XRD)

Abstract

Objective: An Eco-friendly process of Green Synthesis of Copper Nano-particles (CuNPs) is an important aspect in the field of Nanotechnology using alternative feedstock, energy minimization, the design of less toxic and inherently safer chemicals.

Methods: In this study, Copper Nano-Particles were synthesized by using isolated flavonoids to induce the reduction of Cu²⁺ions to CuNPs and also act as a capping and stabilizing agent. The solutions of CuSO₄ and flavonoid were used as stock solutions for the preparation of CuNPs. Aqueous flavonoid(Quercetin) solution was mixed with CuSO₄ solution separately. The reaction mixtures were immediately placed in a hot plate magnetic stirrer at 2000 rpm, 50-60 °C temperature and observed the change in colour of the solution from colourless to coloured solution. The synthesized CuNPs were characterized by various spectral methods for structural analysis of Nano-particles and In vitro and In vivo evaluation for anti-cancer activity.

Results: The results described that the Copper Nano-particles are crystalline and amorphous with an average size of 295.4 nm and highly stable and having good hydrogen peroxide scavenging effect, reducing power and total antioxidant activity with the IC₅₀value of 59.24μg/ml, 24.35μg/ml and16.83μg/ml respectively. The in vitro (HepG2 and MCF-7 cell lines) anti-cancer activity showing good results with IC₅₀ values of 57.56 µg/ml for HepG2 cell line and 56.41 µg/ml for MCF-7 cell line. The in vivo anti-cancer activity also showing good results.

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